Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis
Identifieur interne : 002B76 ( Main/Exploration ); précédent : 002B75; suivant : 002B77Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis
Auteurs : Masayuki Kita [Japon] ; Tomoko Endo [Japon] ; Takehiko Shimada [Japon] ; Takaya Moriguchi [Japon] ; Yutaka Hirata [Japon] ; Shin Hasegawa [États-Unis] ; Mitsuo Omura [Japon]Source :
- Euphytica [ 0014-2336 ] ; 2003.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.
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type="wicri:Area/Main/Curation">002B76</idno><idno type="wicri:Area/Main/Exploration">002B76</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis</title><author><name sortKey="Kita, Masayuki" sort="Kita, Masayuki" uniqKey="Kita M" first="Masayuki" last="Kita">Masayuki Kita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citriculture, National Institute of Fruit Tree Science</s1><s2>Shimizuokitsu, Shizuoka 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Shimizuokitsu, Shizuoka 424-0292</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto</s1><s2>Tsukuba, Ibaraki 305-8605</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Tsukuba, Ibaraki 305-8605</wicri:noRegion></affiliation></author><author><name sortKey="Endo, Tomoko" sort="Endo, Tomoko" uniqKey="Endo T" first="Tomoko" last="Endo">Tomoko Endo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citriculture, National Institute of Fruit Tree Science</s1><s2>Shimizuokitsu, Shizuoka 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Shimizuokitsu, Shizuoka 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citriculture, National Institute of Fruit Tree Science</s1><s2>Shimizuokitsu, Shizuoka 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Shimizuokitsu, Shizuoka 424-0292</wicri:noRegion></affiliation></author><author><name sortKey="Moriguchi, Takaya" sort="Moriguchi, Takaya" uniqKey="Moriguchi T" first="Takaya" last="Moriguchi">Takaya Moriguchi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citriculture, National Institute of Fruit Tree Science</s1><s2>Shimizuokitsu, Shizuoka 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Shimizuokitsu, Shizuoka 424-0292</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto</s1><s2>Tsukuba, Ibaraki 305-8605</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Tsukuba, Ibaraki 305-8605</wicri:noRegion></affiliation></author><author><name sortKey="Hirata, Yutaka" sort="Hirata, Yutaka" uniqKey="Hirata Y" first="Yutaka" last="Hirata">Yutaka Hirata</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>United Graduate School, Tokyo University of Agricultural and Technology</s1><s2>Fuchu, Tokyo 183-0054</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation></author><author><name sortKey="Hasegawa, Shin" sort="Hasegawa, Shin" uniqKey="Hasegawa S" first="Shin" last="Hasegawa">Shin Hasegawa</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street</s1><s2>Albany, CA94710</s2><s3>USA</s3><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Omura, Mitsuo" sort="Omura, Mitsuo" uniqKey="Omura M" first="Mitsuo" last="Omura">Mitsuo Omura</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Citriculture, National Institute of Fruit Tree Science</s1><s2>Shimizuokitsu, Shizuoka 424-0292</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Shimizuokitsu, Shizuoka 424-0292</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Euphytica</title><title level="j" type="abbreviated">Euphytica</title><idno type="ISSN">0014-2336</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Euphytica</title><title level="j" type="abbreviated">Euphytica</title><idno type="ISSN">0014-2336</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Allele</term><term>Bitter taste</term><term>Citrus sinensis</term><term>Citrus unshiu</term><term>Genetic marker</term><term>Glucoside</term><term>Hexosyltransferases</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Saveur amère</term><term>Allèle</term><term>Marqueur génétique</term><term>Citrus unshiu</term><term>Citrus sinensis</term><term>Glucoside</term><term>Hexosyltransferases</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.</div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><noRegion><name sortKey="Kita, Masayuki" sort="Kita, Masayuki" uniqKey="Kita M" first="Masayuki" last="Kita">Masayuki Kita</name></noRegion><name sortKey="Endo, Tomoko" sort="Endo, Tomoko" uniqKey="Endo T" first="Tomoko" last="Endo">Tomoko Endo</name><name sortKey="Hirata, Yutaka" sort="Hirata, Yutaka" uniqKey="Hirata Y" first="Yutaka" last="Hirata">Yutaka Hirata</name><name sortKey="Kita, Masayuki" sort="Kita, Masayuki" uniqKey="Kita M" first="Masayuki" last="Kita">Masayuki Kita</name><name sortKey="Moriguchi, Takaya" sort="Moriguchi, Takaya" uniqKey="Moriguchi T" first="Takaya" last="Moriguchi">Takaya Moriguchi</name><name sortKey="Moriguchi, Takaya" sort="Moriguchi, Takaya" uniqKey="Moriguchi T" first="Takaya" last="Moriguchi">Takaya Moriguchi</name><name sortKey="Omura, Mitsuo" sort="Omura, Mitsuo" uniqKey="Omura M" first="Mitsuo" last="Omura">Mitsuo Omura</name><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name></country><country name="États-Unis"><region name="Californie"><name sortKey="Hasegawa, Shin" sort="Hasegawa, Shin" uniqKey="Hasegawa S" first="Shin" last="Hasegawa">Shin Hasegawa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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